Joint toxicity of cadmium and fenpyroximate on two earthworms: Interspecific differences, subcellular partitioning and biomarker responses.

Cadmium (Cd) and fenpyroximate are common soil contaminants found together in the field, but their combined toxicity to terrestrial invertebrates has not been studied. Therefore, earthworms Aporrectodea jassyensis and Eisenia fetida were exposed into Cd (5, 10, 50 and 100 µg/g) and fenpyroximate (0.1, 0.5, 1, and 1.5 µg/g) and their mixture, and multiple biomarker responses (mortality, catalase (CAT), superoxide dismutase (SOD), total antioxidant activity (TAC), lipid peroxidation (MDA), protein content, weight loss and subcellular partitioning) were determined to estimate health status and mixture effect. MDA, SOD, TAC, and weight loss were significantly correlated with Cd in total internal and debris (p < 0.01). Fenpyroximate altered the subcellular distribution of Cd. It appears that maintaining Cd in a non-toxic form was the earthworms' primary Cd detoxification strategy. CAT activity was inhibited by Cd, fenpyroximate, and their combined presence. BRI values for all treatments indicated a major and severe alteration in earthworm's health. The combined toxicity of Cd and fenpyroximate was greater than the toxicity of either substance alone. According to EAI, all combined treatments exhibited a clear antagonistic effect. In general, the sensitivity of A. jassyensis was greater than that of E. fetida.

Mercury concentration in the feathers of birds from various trophic levels in Fereydunkenar International wetland (Iran).

Mercury (Hg) is one of the main global pollutants that may biomagnify in food nets, especially in wetlands. Birds may be useful in the biomonitoring of Hg in such habitats and may even serve in vivo samples. This paper examined Hg concentration in the feathers of seven bird species foraging on Fereydunkenar International wetland (in 2013). Mean Hg concentrations found ranged from 0.005 ± 0.002 µg g-1 d.w. (dry weight) (Common hoopoe) to 0.38 ± 0.047 µg g-1 d.w. (Greylag goose). Significant differences in Hg concentrations were noted between bird species as well as between trophic levels (one-way ANOVAs, p < 0.001). The decrease in mean Hg concentration in feathers was as follows: Greylag goose > Northern pintail ≥ Gadwall ≥ Mallard > Eurasian bittern ≥ Little bittern > Common hoopoe. The position in the trophic chain significantly influenced Hg concentrations, which were the highest in omnivorous species. Hg concentrations may also depend on migration routes and breeding habitats, but the evaluation of the exposure exceeds the ambit of this paper. The Hg concentrations found generally were low, lower than the safe thresholds reported in the literature.

Mercury levels in avian feathers from different trophic levels of eight families collected from the northern region of Iran.

Mercury levels were determined in feathers from 83 birds belonging to 18 species (eight families), all collected from the northern region of Iran. Mercury levels were evaluated in relation to taxonomic affiliation and feeding strategies. Mercury levels in the feathers were between 0.05 ± 0.01 and 1.10 ± 0.15 µg g(-1) dry weight, and there was a significant effect of taxonomic groups (p < 0.05). The highest mercury levels were found in Accipitridae, and mercury was not detected in the family Upupidae. The pattern for mercury levels was Accipitridae > Pelecanidae > Sternidae > Ardeidae > Anatidae > Rallidae > Phasianidae (p < 0.05). Significant differences (p < 0.05) in the mean mercury levels were found among species as a function of feeding method and trophic level. Mercury levels were highest in the carnivorous species and lowest in the herbivorous species. Mercury levels in feathers of birds in this study were generally below the thresholds reported to affect reproduction.

Relation between Neonatal Icter and Gilbert Syndrome in Gloucose-6-Phosphate Dehydrogenase Deficient Subjects.

BACKGROUND AND AIM: The pathogenesis of neonatal hyperbilirubinemia hasn't been completely defined in Gloucose-6-Phosphate Dehydrogenase (G6PD) deficient newborns. The aim of this study was to detect the relationship between Gilbert's syndrome and hyperbilirubinemia in Gloucose-6-Phosphate Dehydrogenase (G6PD) deficient neonates. MATERIALS AND METHODS: This case-control study was conducted in Amirkola pediatrics teaching hospital, Babol, Iran. A total number of one hundred four infants were included in the study (51 infants with neonatal jaundice and Gloucose-6-Phosphate Dehydrogenase (G6PD) deficiency admitted to phototherapy or transfusion were selected as the case group and 53 infants with Gloucose-6-Phosphate Dehydrogenase (G6PD) deficiency admitted for other reasons than jaundice were selected as the control group). Exclusion criteria were ABO or Rh incompatibility or other reasons that made Coombs test positive, sepsis, hepatosplenomegaly, metabolic diseases, medical treatment and phototherapy. The promoter and coding regions of Uridine diphosphate Glucuronosyl Transferase 1A1 (UGT1A1) of genomic DNA were amplified by polymerase chain reaction (PCR) isolated from leukocytes. We used chi-square test and t-test to compare cases and controls. RESULTS: Distribution of Gilbert genome was not significantly different between the two groups; among cases, 33.3% were homozygote, 35.3% heterozygote, and 31.4% normal. Among controls, 22.6% were homozygote, 34% heterozygote, and 43.4% normal (p-value=xxx). Hyperbilirubinemia family history didn't differ significantly between these two groups. CONCLUSIONS: We showed that in Gloucose-6-Phosphate Dehydrogenase (G6PD) deficient neonates, there was no significant association between Gilbert's syndrome (promoter polymorphism) and hyperbilirubinemia.